Biosensors
Application of Biosensors to Evaluate Influenza Strains
The molecular imprinting technique is a very versatile method that has been used in diverse applications including molecular sensing, antibody screening, drug delivery and protein and virus classication. Molecularly Imprinted Polymer (MIP) can be created via polymerizing a highly crosslinked matrix in the presence of a template. This can consist of individual molecules or mixtures thereof, proteins or even larger species including viruses. The imprinting process therefore results in a “negative” image of the template being imprinted into the polymer itself. Removing the original template from the polymer reveals accessible cavities on its surface that retain selective molecular information regarding both morphological and functional properties of the template. MIPs are both rapid and relatively inexpensive to produce and offer signifcant potential for use in a screening environment. The unique shape selectivity defned by the original template leads to an MIP that can potentially allow us to differentiate between even subtly different molecular systems
In this work, a molecular imprinting strategy has been employed as screening protocol for different influenza A subtypes, namely H5N1, H5N3, H1N1, H1N3 and H6N1. Molecularly imprinted polymers for each of these subtypes lead to appreciable sensor characteristics on a quartz crystal microbalance leading to detection limits as low as 105 particles per ml. Selectivity studies indicate that each virus is preferably incorporated by its own MIP. Recognition in most cases is dominated by the neuraminidase residue rather than the hemagglutinin. Multivariate analysis shows that the sensor responses can be correlated with the differences in hemagglutinin and neuraminidase patterns from databases. This allows for virus subtype characterization and thus rapid screening.
In this work, a molecular imprinting strategy has been employed as screening protocol for different influenza A subtypes, namely H5N1, H5N3, H1N1, H1N3 and H6N1. Molecularly imprinted polymers for each of these subtypes lead to appreciable sensor characteristics on a quartz crystal microbalance leading to detection limits as low as 105 particles per ml. Selectivity studies indicate that each virus is preferably incorporated by its own MIP. Recognition in most cases is dominated by the neuraminidase residue rather than the hemagglutinin. Multivariate analysis shows that the sensor responses can be correlated with the differences in hemagglutinin and neuraminidase patterns from databases. This allows for virus subtype characterization and thus rapid screening.
Article weblink: http://pubs.rsc.org/en/Content/ArticleLanding/2013/TB/c3tb00027c

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